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Image Search Results
Journal: Virologica Sinica
Article Title: Establishment of Murine Infection Models with Biological Clones of Dengue Viruses Derived from a Single Clinical Viral Isolate
doi: 10.1007/s12250-020-00229-y
Figure Lengend Snippet: DENV-2 specific adaptive immune responses elicited by DENV-2 8H2-7-LP and DENV-2 1D4-5-SP infection. At 40 days post infection, FRNT (Foci Reduction Neutralization Test) A and IFN-γ Elispot assay ( B ) were performed with mouse sera and splenocytes, respectively. Reference strain DENV-2 16681 was used in FRNT assay, peptides and proteins derived from DENV-2 16681 (D2-NS1 265, D2-NS1, D2-E80) and Zika virus (Z-NS3 43, Z-NS3 291) were included in Elispot assay to measure specific and cross-reactive responses; n = 4. Significance of the differences was calculated with t test, *** P < 0.001.
Article Snippet: After depleting erythrocytes, cells were re-suspended in 10% FBS/PRMI-1640 and added to
Techniques: Infection, Neutralization, Enzyme-linked Immunospot, Derivative Assay, Virus